Part:BBa_K2267040

LuxR-I-Plux-GFP

This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.

Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.

https://parts.igem.org/Part:BBa_K2267026  is Control， 

part1: https://parts.igem.org/Part:BBa_K2267043

part3: https://parts.igem.org/Part:BBa_K2267041

part4: https://parts.igem.org/Part:BBa_K2267042

part6: https://parts.igem.org/Part:BBa_K2267044

part8: https://parts.igem.org/Part:BBa_K2267045

result

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 837
  Illegal NheI site found at 860
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 928
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1452
  Illegal BsaI.rc site found at 2179